in vitro mechanics
Measurement | Muscles studied | Parameters assessed |
Maximum tetanic force | EDL, Soleus, Diaphragm | Force generating capacity |
Eccentric Contraction | EDL, Soleus, Diaphragm | Susceptibility to contractile damage |
Force-frequency profile | EDL, Soleus, Diaphragm | Calcium handling shifts |
Fatigue index | EDL, Soleus, Diaphragm | Fiber type and metabolic shifts |
Passive force | EDL, Soleus, Diaphragm | Fibrotic composition |
in situ mechanics
Measurement | Muscles studied | Parameters assessed |
Maximum tetanic force | Tibialis Anterior | Force generating capacity |
Eccentric Contraction | Tibialis Anterior | Susceptibility to contractile damage |
Force-frequency profile | Tibialis Anterior | Calcium handling shifts |
Whole animal manipulations
Measurement | Muscles studied | Parameters assessed |
Hindlimb suspension | Soleus, gastrocnemius, plantaris | Model for disuse atrophy |
Free-wheel running | Whole animal | Voluntary exercise |
Weighted wheel running | Whole animal | Voluntary training |
Downhill treadmill running | Whole animal, diaphragm | Induction of contractile damage |
Treadmill Run to exhaustion | Whole animal | Endurance |
TD NMR | Whole animal | Body Composition |
Plethysomography (with DD Fuller Laboratory) | Whole animal, diaphragm | Respiratory Function |
Dye uptake Studies
Measurement | Muscles studied | Parameters assessed |
Procion Orange | In vitro mechanics muscles | Sarcolemmal susceptibility to injury |
Evan’s Blue Dye | Whole animal | Ongoing membrane damage and necrosis |
Other
Measurement | Muscles studied | Parameters assessed |
Blood – Creatine Kinase levels | Whole animal | Ongoing membrane leakiness |
Blood – Glucose | Whole animal | Metabolic homeostasis |