Passive Mechanical Protocol

Passive mechanical protocol graph

Figure 2. Passive mechanical protocol with example data from a C57 EDL muscle. (A) After preconditioning stress relaxation is held for 2 minutes with Squares representing maximum stress (dynamic stiffness), and circles representing stress at 2 minutes used to (elastic stiffness). (B) Strains of 2.5- 15%, with a ramp stretch of 1 fiber length/second and held for 2 minutes. (C) Stiffness is determined from a quadratic fit to the stress strain curves taking the tangent modulus at 10% strain for both dynamic and elastic stiffness. From Smith and Barton, 2014.

Related Literature

Moorwood, C., T. Philippou, J. Spinazzola, B. Keyser, E.J. Macarak, and E.R. Barton. (2014) Absence of γ-sarcoglycan alters the response of p70S6 kinase to mechanical perturbation in murine skeletal muscle. Skeletal Muscle, 4: 13. PMCID: PMC4095884

Smith, L.R. and E.R. Barton. (2014) Collagen content does not contribute to passive mechanics properties in fibrotic skeletal muscle of mdx mice. Am J. Physiol Cell, 306 (10): C889-898. PMCID: PMC4024713

Zou, Y., D. Zwolanek, Y. Izu, S. Gandhy, G. Schreiber, K. Brockmann, M. Devoto, Z. Tian, Y. Hu, G. Veit, M. Meier, J. Stetefeld, D. Hicks, V. Straub, N.C. Voermans, D.E. Birk, E.R. Barton, M. Koch, C.G. Bonnemann. (2014) Recessive and dominant mutations in COL12A1 cause a novel EDS/myopathy overlap syndrome in human and mice. Hum Mol Gen, 23(9):2339-52. PMCID: PMC3976332